Brazilian Journal of Otorhinolaryngology (English Edition) Brazilian Journal of Otorhinolaryngology (English Edition)
Braz J Otorhinolaryngol 2017;83:670-6 DOI: 10.1016/j.bjorl.2016.09.003
Original article
Identification of key genes involved in nasopharyngeal carcinoma
Identificação dos principais genes envolvidos no carcinoma nasofaríngeo
Xue Jiang, Lichun Feng, Baoqiang Dai, Liping Li, Weiwei Lu,
Cangzhou Central Hospital, Department of Otorhinolaryngology, Cangzhou, Hebei, China
Received 18 May 2016, Accepted 11 September 2016
Abstract
Introduction

Nasopharyngeal carcinoma is the most common cancer originating from the nasopharynx.

Objective

To study the mechanisms of nasopharyngeal carcinoma, we analyzed GSE12452 microarray data.

Methods

GSE12452 was downloaded from the Gene Expression Omnibus database and included 31 nasopharyngeal carcinoma samples and 10 normal nasopharyngeal tissue samples. The differentially expressed genes were screened by ANOVA in the PGS package. Using the BiNGO plugin in Cytoscape and pathway enrichment analysis in the PGS package, functional and pathway enrichment analyses were performed separately to predict potential functions of the differentially expressed genes. Furthermore, Transcription factor-differentially expressed gene pairs were searched, and then the transcription factor-differentially expressed gene regulatory network was visualized using Cytoscape software.

Results

A total of 487 genes were screened as differentially expressed genes between the nasopharyngeal carcinoma samples and the normal nasopharyngeal tissue samples. Enrichment analysis indicated that PTGS2 was involved in the regulation of biological process and small cell lung cancer. ZIC2 and OVOL1 may function in nasopharyngeal carcinoma through targeting significantly up-regulated genes (such as PTGS2, FN1, CXCL9 and CXCL10) in the Transcription factor-differentially expressed gene regulatory network (e.g., ZIC2→PTGS2 and OVOL1→CXCL10).

Conclusion

PTGS2, FN1, CXCL9, CXCL10, ZIC2 and OVOL1 might play roles in nasopharyngeal carcinoma.

Resumo
Introdução

O carcinoma nasofaríngeo é o câncer mais comum originário da nasofaringe.

Objetivo

Estudar os mecanismos do câncer de nasofaringe; dados do microarray GSE12452 foram analisados.

Método

GSE12452 foi obtido da base de dados Gene Expression Omnibus e inclui 31 amostras de carcinoma nasofaríngeo e 10 amostras de tecido nasofaríngeo normal. Os genes diferencialmente expressos foram analisados por ANOVA no kit PGS. Usando o plugin BiNGO no Cytoscape e análise de enriquecimento da via no kit PGS, análises de enriquecimento funcional e da via foram realizadas separadamente para prever as potenciais funções dos genes diferencialmente expressos. Além disso, os pares Fator de Transcrição - genes diferencialmente expressos foram pesquisados e em seguida a sua rede reguladora foi visualizada usando o programa Cytoscape.

Resultados

Um total de 487 genes foram analisados como genes diferencialmente expressos entre as amostras de carcinoma nasofaríngeo e amostras de tecido nasofaríngeo normal. A análise de enriquecimento indicou que PTGS2 estava envolvido na regulação do processo biológico e câncer pulmonar de pequenas células. ZIC2 e OVOL1 podem funcionar no carcinoma nasofaríngeo almejando-se de maneira significativa os genes suprarregulados (como o PTGS2, FN1, CXCL9 e CXCL10) na rede reguladora de fator de transcrição - genes diferencialmente expressos (p.ex., ZIC2→PTGS2 e OVOL1→CXCL10).

Conclusão

PTGS2, FN1, CXCL9, CXCL10, ZIC2 e OVOL1 podem desempenhar alguns papéis no carcinoma nasofaríngeo.

Keywords
Nasopharyngeal carcinoma, Differentially expressed genes, Enrichment analysis, Regulatory network
Palavras-chave
Carcinoma nasofaríngeo, Genes diferencialmente expressos, Análise de enriquecimento, Rede reguladora
Braz J Otorhinolaryngol 2017;83:670-6 DOI: 10.1016/j.bjorl.2016.09.003